FineFIX

Overview Why FineFIX Applications FAQ's

FineFIX: the new formaline-free fixative

The search for the ideal fixative is on

The ideal fixative should present a low level of toxicity, produce optimal H&E, IHC and histochemical staining and allow the recovery of DNA, RNA, Protein for molecular analysis.
Milestone has taken advantage of its know-how from over 25 years of combined experience in the field of microwave enhanced organic chemistry and microwave applications in histology to develop a new fixative - FineFIX, formulated for conventional and microwave enhanced fixation. This highly efficient formalin-free fixative is ideal for routine histological applications and molecular analysis.

What is FineFIX ?

FineFIX is a patented formalin-free, water-based concentrate. When diluted with ethanol, its formulation of low toxicity additives overcomes the drawbacks commonly associated with the use of pure ethanol or ethanol based fixatives, e.g., significant tissue shrinkage, vacuolization and pyknotic nuclei. FineFIX also provides optimal preservation of tissue antigens, nuclear and cytoplasmic morphology and reduced lysis of red blood cells with preservation of the cytoplasmic membranes.
FineFIX working solution is prepared by mixing 1 part of FineFIX concentrated to 3 parts of ethanol (98%)
The ethanol concentration in the working solution of FineFIX is approximately 70%. This concentration was found to produce good histology and to allow optimal recovery of DNA/RNA and proteins, sufficient for several downstream molecular analyses.

Handbook of guidelines for the correct use of FineFIX

FineFIX is supplied with a handbook of guidelines to enable users to immediately operate with this fixative, avoiding any trial and error experiments.
Guidelines for a smooth transition to a formalin-free laboratory.