Hours, not days!
Rapid bone marrow processing for same-day diagnosis
Tissue processors
Histos 3, Histos 5, KOS
Method
Rapid fixation (formalin), decalcification (EDTA, formic acid, etc.) and processing by microwave energy enhanced protocols. Standard embedding, cutting, H&E staining.
Typical protocols
 Bone marrow specimens: 1-2 mm
From 1 to 45 cassette
| |
Minutes |
Temp. °C |
| Soaking in formalin |
15 |
Ambient |
| Fixation in formalin (microwave) |
15 |
50 |
| Decalcification (formic acid) |
20-40 |
50 |
| Decalcification (EDTA) |
60-120 |
50 |
| Tissue processing (eth+iso) |
40 |
65 |
| Impregnation in wax |
40 |
65-70 |
Total time
2-3 hours with formic acid
3-4 hours with ETDA
High quality results
 |
| Bone marrow, EDTA, H&E x 100 |
|
