Hours, not days!
Processing of prostate whole mount in less than 7 hours, including fixation
Tissue processors
Pathos, Pathos Delta, Histos 5
Tissues
Prostate whole mount up to 5-7 mm thickness
Method
Fixation: formalin
Processing: Ethanol - Isopropanol - Wax
Rapid fixation and processing by microwave energy enhanced protocols. Standard embedding, cutting, H&E, IHC staining
Cassette type: SuperMEGA with special insert
Typical protocols
 Prostate whole mount 4-5 mm thickness
| |
Minutes |
Temp. °C |
Fixation
|
60 |
50 |
| Tissue processing (Ethanol+Isopropanol) |
130 |
68 |
| Impregnation in Wax |
215 |
65-70 |
Total time
Approx 5h 35'
High quality results
 |
 |
 |
Prostate, H&E.
Low power macro with area of interest |
Nodular Hyperplasia H&E x100 |
p53 x600 |
|
