Confirmation of frozen sections in minutes, not days!
Microwave rapid processing for permanent slides of transplant biopsies
Tissue processors
Histos 3, Histos 5, KOS
Tissues
Heart, kidney, liver, lung, skin, etc.
Method
Fixation: formalin
Processing: Ethanol - Isopropanol - Wax
Rapid fixation and processing by microwave energy enhanced protocols. Standard embedding, cutting, H&E, IHC staining
Typical protocols
Needle biopsies: 1 mm thickness
| |
Minutes |
Temp. °C |
| Soaking in formalin |
15 |
Ambient |
| Fixation in formalin (microwave) |
15 |
50 |
| Tissue processing (eth+iso) |
40 |
65 |
| Impregnation in wax |
40 |
65-70 |
Total time
50 minutes (including fixation)
High quality results
 |
 |
 |
| Liver, H&E x100 |
Kidney Silver Stain |
Skin, H&E x100 |
|