JFC
Solution
Patented JFC solution (ethanol + isopropanol + long chain
hydrocarbon) for consistent and reliable processing of fatty tissues.
JFC Solution: FAQ
What is
the composition of the one-step dehydrating/clearing solution (JFC)? JFC
solution is a patented product consisting of a mixture of ethanol, isopropanol,
and a long chain hydrocarbon.
Can I use other reagents instead of JFC for the histoprocessing?
Yes. The PATHOS and Histos microwave processors are not closed systems
for reagents. They were developed to allow the user to choose the proprietary
JFC solution, or ethanol-isopropanol, or a combined use of ethanol-JFC, for
use as follows:
- Ethanol-Isopropanol-Wax
Ethanol-isopropanol protocol is recommended as the standard method for
histoprocessing small to medium-large biopsies, provided that the fat
content of the tissue blocks is kept to a minimum. It is a very cost-effective
protocol for general histoprocessing. This is the recommended method for
processing of renal biopsies.
- Ethanol-JFC-Wax
Ethanol-JFC is for use with tissues where overnight fixation of tissue
is uncertain, and for partially fixed tissues. In this instance, the ethanol
acts as a post-fixative step, since JFC is not a fixative. This protocol
is especially suited for routine use and for very fatty tissues, such
as breast. The ethanol step will extend the reuse life of the JFC.
- JFC-Wax
JFC solution is strongly recommended for tissues known to be well fixed,
generally at least overnight, and is of major benefit when dealing with
tissues that contain high lipid content, such as larger pieces of breast
and brain.
How many times can I re-use the JFC solution?
It can be used at least twice, and possibly further, depending
on the quantity and size of samples processed, and whether a preceding microwave
step in ethanol is used. It is essential, if you intend to reuse again,
to make sure that the container is covered to prevent loss of reagent vapors,
and to prevent absorption of atmospheric moisture.
Can I dispose of JFC solution down the drain? No.
Due to the properties of the hydrocarbon (not miscible with water in pure
form), it may accumulate and therefore increase risks of flammability in
the drainage system. Dispose of JFC solution in accordance with disposal
instructions from your local environmental protection agency.
Can I recycle the JFC solution? No, as the
ratio of the individual components is not disclosed. However, if the recycling
unit is of a high standard, the individual components may be used for other
applications (e.g., ethanol use in staining machines).
What is the temperature of the JFC solution used?
68°C.
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